Studying Microbiote Competition and Skin Interaction Using Organotypic 3D Skin Models
ثبت نشده
چکیده
The skin surface varies topographically owing to regional differences in skin anatomy and, according to culture-based studies, these regions are known to support distinct sets of microorganisms [1]. Regions with higher temperature and humidity encourage growth of Gram-negative bacilli, coryneforms and Staphylococcus aureus, whereas areas with high density of sebaceous glands encourage growth of lipophilic microorganisms such as Malassezia spp or facultative anaerobes such as Propionibacterium acnes. Because humans sebum contain much more quantities of triglyceride than other mammals to support skin protection, Propionibacterium acnes is particularly present in greater abundance in human skin. When skin homeostasis is disturbed, commensal bacteria could become pathogens and could contribute to tissue damage as for acne and also rosacea [2], dandruff [3], atopic dermatitis [4] or wound healing [5].
منابع مشابه
Studying Microbiote Competition and Skin Interaction Using Organotypic 3D Skin Models
The skin surface varies topographically owing to regional differences in skin anatomy and, according to culture-based studies, these regions are known to support distinct sets of microorganisms [1]. Regions with higher temperature and humidity encourage growth of Gram-negative bacilli, coryneforms and Staphylococcus aureus, whereas areas with high density of sebaceous glands encourage growth of...
متن کاملStudying the Human Skin Microbiome Using 3D In Vitro Skin Models
Labskin is a commercial 3D in vitro skin model based on the original Leeds Model specifically developed for studying interactions between the skin and its microbiome. Further development of the microbiology protocols for growth, preparation, inoculation, and recovery have enabled Labskin to be used for a wide variety of applications in skin microbiology. The method of construction of the model ...
متن کاملThe use of skin models in drug development.
Three dimensional (3D) tissue models of the human skin are probably the most developed and understood in vitro engineered constructs. The motivation to accomplish organotypic structures was driven by the clinics to enable transplantation of in vitro grown tissue substitutes and by the cosmetics industry as alternative test substrates in order to replace animal models. Today a huge variety of 3D...
متن کاملGeneration of Genetically Modified Organotypic Skin Cultures Using Devitalized Human Dermis.
Organotypic cultures allow the reconstitution of a 3D environment critical for cell-cell contact and cell-matrix interactions which mimics the function and physiology of their in vivo tissue counterparts. This is exemplified by organotypic skin cultures which faithfully recapitulates the epidermal differentiation and stratification program. Primary human epidermal keratinocytes are genetically ...
متن کاملContribution of In Vivo and Organotypic 3D Models to Understanding the Role of Macrophages and Neutrophils in the Pathogenesis of Psoriasis
Psoriasis, a common chronic immune-mediated skin disease, is histologically characterized by a rapid keratinocyte turnover and differentiation defects. Key insights favor the idea that T cells are not the only key actors involved in the inflammatory process. Innate immune cells, more precisely neutrophils and macrophages, provide specific signals involved in the initiation and the maintenance o...
متن کامل